Skip to main content
Fig. 2 | Journal of Ovarian Research

Fig. 2

From: CRISPR/Cas9-mediated activation of NR5A1 steers female human embryonic stem cell-derived bipotential gonadal-like cells towards a steroidogenic cell fate

Fig. 2

NR5A1 induction upregulates steroidogenesis markers but downregulates the aromatase enzyme CYP19A1. A RT-qPCR analysis of markers associated with steroidogenesis showed that NR5A1 induction upregulated the markers STAR, CYP11A1, CYP17A1, HSD3B2 and HSD17B1, but downregulated the aromatase enzyme CYP19A1. Data are reported as mean ± SEM, n=3 biological replicates. Two-way ANOVA; 0.12 (ns), 0.033 (*), 0.002 (**), < 0.001 (***). B Confocal images of immunofluorescence staining showed expression of steroidogenic markers STAR and CYP19A1 in non-induced (-DOX -TMP) and induced (+DOX +TMP) conditions at day 10 of gonadal differentiation. Images were taken with a ×40 objective. Scale bars 50 µm. d, day of differentiation; DOX, doxycycline hyclate; TMP, trimethoprim

Back to article page

Journal of Ovarian Research

ISSN: 1757-2215

Contact us