Fig. 4

MDHB protects KGN cells against TBHP‑induced oxidative stress. A ROS staining (green) were performed to detected the protective effects of MDHB on TBHP‑induced oxidative stress in KGN cells. B MitoSOX staining (red) were performed to detected the protective effects of MDHB on TBHP‑induced oxidative stress in KGN cells. C JC-1 staining was detected for mitochondrial membrane potential, where green represents JC-1 monomers indicating low potential, and red represents JC-1 aggregates indicating high potential. D-F The analysis of relative fluorescence intensity for ROS, MitoSOX and JC-1 staining. G The effects of TBHP or MDHB treatment on ATP production. All data were presented as the Means ± SD, *P < 0.05, **P < 0.01