Fig. 1

Rosiglitazone treatment contributed to the establishment of a non-metabolic PCOS mouse model. (A) Representative estrous cycles of mice in the control, DHEA and DHEA + rosiglitazone groups. (B) Quantitative analysis of estrous cycles (data are presented as the medians with interquartile ranges, n = 5 mice per group). (C) Haematoxylin and eosin staining of representative ovaries. The corpora luteum are indicated by asterisks. Images are representative of three independent experiments with similar results. Scale bar: 250 μm. (D) Quantitative analysis of corpora luteum (n = 5 mice per group). (E-H) Serum levels of testosterone, estradiol, LH and FSH in mice in the control, DHEA and DHEA + rosiglitazone groups (n = 5 mice per group). (I) The ratio of LH/FSH. (J and K) GTT and area under the curve of the GTT (n = 5 mice per group). (L) ITT (n = 5 mice per group). The number of biological replicates is indicated as ‘n’. P, proestrus; E, estrous; M/D, metestrus/diestrus. GTT, glucose tolerance test; ITT, insulin tolerance test. Data are presented as the means ± SEMs. For (J and L) *P < 0.05; ***P < 0.001 versus the control group. #P < 0.05; ###P < 0.001 versus the DHEA + rosiglitazone group