Fig. 2

SK treatment reduces the promoting effect of OC cell-derived exo on M2 polarization of macrophages. Exo from SKOV3 and A2780 cells, either with or without 5 µM SK treatment for 48 h, were collected, which were designated to OC exo and SK OC exo, respectively. A, morphology of isolated OC exo or SK OC exo determined under TEM; B, particle size distribution and concentration of isolated OC exo or SK OC exo examined by NTA; C, protein expression of exo marker proteins CD9, CD63 and CD81 and endoplasmic reticulum marker Calnexin in the isolated OC exo or SK OC exo determined by WB analysis; D, successful uptake of DiO-labeled exo by THP-1 cells observed under the fluorescence microscope. THP-1 cells were stimulated with 150 nM PMA for 24 h to differentiate into M0 macrophages, followed by treatment with PBS, OC exo or SK OC exo. E-F, percentage of CD163- (E) and CD206-(F) positive THP-1 cells examined by flow cytometry; G, production of IL-10 in the culture supernatant of THP-1 cells examined using ELISA; H, mRNA expression of CD163 and CD206 in THP-1 cells determined by RT-qPCR. Three biological replicates were performed. Differences were analyzed by the one-way or two-way ANOVA. *p < 0.05