Fig. 3

SK treatment reduces GAL3 protein level in OC exo. A, intersections of molecular targets of SK predicted from Super-PRED system, proteins enriched in OC cell (OVCAR-3 and IGROV1)-derived exo obtained from the ExoCarta system, and differentially expressed genes between M0 and M2 macrophages obtained by analyzing the GEO GSE159112 dataset; B, protein-protein interaction between the 12 intersections analyzed using the STRING system; C, a positive correlation between LGALS3 and M2 macrophage polarization in OC predicted using the TIMER system; D, correlations between LGALS3 expression and CD163 and CD206 expression in OC predicted using the GEPIA system; E, GAL3 protein level in OC exo and SK OC exo determined by WB analysis (the band intensity of GAL3 in OC exo was used for normalization); F, GAL3 protein level in THP-1 cells after OC exo or SK OC exo treatment examined by WB analysis. THP-1 cells treated with SK OC exo were further transfected with OE-LGALS3 or OE-NC. G, GAL3 protein level in THP-1 cells determined by WB analysis; H-I, percentage of CD163 (H)- and CD206 (I)-positive THP-1 cells determined by flow cytometry; J, production of IL-10 in the culture supernatant of THP-1 cells examined using ELISA; K, mRNA expression of CD163 and CD206 in THP-1 cells determined using RT-qPCR. Three biological replicates were performed. Differences were analyzed by the two-way ANOVA. *p < 0.05