Fig. 4

GAL3 promotes nuclear translocation of β-catenin to induce M2 polarization of macrophages. A, nuclear and cytoplastic β-catenin levels in THP-1 cells after OC exo or SK OC exo treatment determined by WB analysis. THP-1 cells treated with SK OC exo and transfected with OE-LGALS3 were further treated with the β-catenin inhibitor FH535 or DMSO. B-C, percentage of CD163 (B)- and CD206 (C)-positive THP-1 cells examined by flow cytometry; D, production of IL-10 in the culture supernatant of THP-1 cells examined using ELISA; E, mRNA expression of CD163 and CD206 in THP-1 cells determined by RT-qPCR. Three biological replicates were performed. Differences were analyzed by the two-way ANOVA. *p < 0.05