Table 3 Predictive accuracy of serum E₂ and β-hCG concentrations on day 12 following FET with NCs

Prediction of clinical pregnancy of cleavage embryos

Day 12 E₂ levels (pg/mL)

0.978 (0.972–0.984)

P < 0.001

0.801 (0.756–0.846)

129.25 mIU/ml

74.45%

71.28%

Day 12 β-hCG levels (mIU/mL)

0.979 (0.973–0.984)

P < 0.001

0.941 (0.923–0.959)

156.60 mIU/ml

84.20%

95.74%

Prediction of clinical pregnancy of blastocyst embryos

Day 12 E₂ levels (pg/mL)

0.986 (0.983–0.989)

P < 0.001

0.762 (0.726–0.799)

174.45 mIU/ml

63.77%

77.84%

Day 12 β-hCG levels (mIU/mL)

0.988 (0.986–0.991)

P < 0.001

0.924 (0.907–0.941)

217.70 mIU/ml

81.56%

94.01%