Fig. 1

The ferroptosis pathway was involved in 4HC-treated GCs. (A) Scatter plot (red, upregulated; green, downregulated), (B) KEGG enrichment analysis and (C) GO enrichment items for differentially expressed genes (DEGs) of RNA-seq in COV434 cells in response to 4HC treatment compared with control. (D) Venn plots and STRING analysis the gene intersections taken between the DEGs, oxidative stress- and ferroptosis- gene sets. (E) Venn plots and STRING analysis the gene intersections taken between the DEGs, oxidative stress- and mitochondrial- gene set. (F) Relative expression of the HO-1 and GPX4 mRNAs and (G) proteins in COV434 and KGN cells treated with 4HC (15 µM and 25 μm, respectively) for 24 h. Data are presented as the means ± SEM from more than three independent experiments; *p < 0.05, ***p < 0.001 and ****p < 0.001 for the indicated comparisons