Fig. 2

Ferroptosis inhibitor reversed 4HC-induced cytotoxicity. (A) Dose- and time-response curve used to estimate IC50 values of 4HC in COV434 and (B) KGN cells, accompanied by increased toxicity. (C) Morphological changes of COV434 and (D) KGN cells treated with 4HC with or without Fer-1 for 24 h. (E) Dose-response curve of 4HC was preincubated with or without Fer-1 (20 µM) for 24 h in COV434 and (F) KGN cells. Cell viability and toxicity was expressed as percentage with respect to control and 4HC at different concentrations. Each point represents the mean of quadruplicates. Scale bars = 200 μm. ****p < 0.0001 for the indicated comparisons