Fig. 8

Callithrix jacchus embryo development in vitro and morphological appearance of the respective developmental stages. A Timeline of embryo development from in vivo matured oocytes (recovered at MII stage, starts from embryo day 0 (ED0) or in vitro matured oocytes (recovered at the germinal vesicle (GV) stage, starts from 28–30 h IVM). B Proportion of embryos with blastocoel first visible on ED8, ED9 and ED10 analysed in 22 blastocysts obtained during extended culture of IVM oocytes in FSH-priming group (n = 9). C Morphological appearance of the respective developmental stages: immature cumulus-oocyte complex (a); COC after IVM, with expanded cumulus surrounding the oocyte (b); fertilised oocyte with visible 2 PB’s (black arrows) and 2 pn (white arrowheads) (c); 2-cell embryo (d); 4-cell embryo (e); 8-cell embryos (f); 12-cell embryo (g), initiation of compaction (h); compacted morula (i); morula-blastocyst transition (j); blastocyst with a visible zona pellucida cut (black arrowhead) after artificial hatching performed at morula stage (k); hatching blastocyst (l)