Fig. 3

Functional role of CENP-O in regulating ovarian cancer cell cycle and clonal formation in vitro. (A) A colony formation assay was performed using SK-OV-3 cells transduced with the CENPO shRNA lentivirus. (B) Number of SK-OV-3 cell colonies that formed following transduction with the CENPO shRNA lentivirus. (C) Flow cytometry was used to analyze cell populations of different stages of the cell cycle. (D) The proportion of ES-2 cells in different cell cycles. (E) β-galactosidase activity assay was performed using SK-OV-3 and ES-2 cells transduced with the CENPO shRNA lentivirus. (Significant differences between two groups were analyzed using Student’s t-test. Significance was indicated as follows: p ≤ 0.05 (*); p ≤ 0.01 (**); p ≤ 0.001 (***); p ≤ 0.0001 (****))