Fig. 2

Decreased ovarian function and reproduction in four-month-old cKO(HE) female mice. A litter size and (B) pup weight in the controls (n = 4) and cKO(HE) mice (n = 6). C-D ovarian volume and ovarian mass in the control (n = 4) and cKO(HE) (n = 3) mice. E–F gravid uterus at E18.5 and E11.5 in the control (n = 5) and cKO(HE) (n = 6) mice. G. representative H&E-stained sections of ovaries (n = 3). H the number of primordial, primary, second, and preantral follicles in controls (n = 3) and cKO(HE) (n = 3) mice. I BDNF protein expression in ovaries was measured by ELISA (n = 3). J FSH protein expression in ovaries was measured by ELISA in the controls (n = 5) and cKO(HE) (n = 5) mice. Data are presented as mean ± SD. NS indicates not significant. * Indicates P < 0.05. ** indicates P < 0.01