Fig. 6

The number of PGCs and the expression of Cyclin D1, a cell proliferation biomarker. A the morphology of embryos at E11.5 (n = 3). B alkaline phosphatase staining of genital ridges and PGC counts at E11.5. The brown spots indicate PGCs cKO(HE) mice (n = 3) and the controls (n = 3). C Representative immunofluorescent staining images for Cyclin D1 and the quantitive analysis of Cyclin D1 expression in embryos’ genital ridges cKO(HE) mice (n = 4) and the controls (n = 3)