Fig. 4

Isolation of Plasma EVs by Size Exclusion Chromatography. EVs were isolated using size-exclusion chromatography from plasma of patients with early-stage HGSC (n = 16) and benign gynecological disease (n = 16). EV concentration and mean diameter were estimated using nanoparticle tracking analysis. EV isolations from HGSC and Benign donors displayed comparable (A) particle concentrations and (B) average mean diameter of particles detected. Blue horizontal lines represent mean. NTA estimated a mean diameter of 120 µm for plasma EVs. (C, D) Topographical analysis of HGSC EVs by atomic force microscopy detected EV-like particles with mean diameter of 134 nm. (C) Insert a representative line trace of a single particle, displaying a dome like structure. D Data represented as mean and standard deviation using box plot with inner quartiles shown. E Enrichment Analyses of Reactome and GO Biological Process annotations in comparison to total proteins identified by GPF-DIA MS/MS