Fig. 4

Western blot used to detect the expression of apoptosis-related protein in each group. Protein expression levels of Cleaved Caspase-3, Caspase-3, Cleaved Caspase-9, Caspase-9, Bax and BCL-2 were detected in ADSCs-/ADSCs-Exos-containing-treated groups (A) and different treatment groups (C). Quantitative analysis of protein expression indicated that the expression level of Cleaved Caspase-3, Cleaved Caspase-9 and Bax was reduced, whereas BCL-2 expression was increased in ADSCs-treated group, ADSCs-Exos-treated group (B), fragment-ADSCs group and fragment-ADSCs-Exos group (D). (* versus the normal group, # versus the POI group, & comparison between two groups; *, #, & P < 0.05; **, ##, && P < 0.01; ***, ###, &&& P < 0.001; n = 3 per group)