Fig. 12
DCHS1 was involved in cell proliferation, migration and EMT. A. DCHS1 was downregulated in UCEC analyzed by GEPIA2.0. B-D. The differential expression of DCHS1 in GEO17025, GSE63678 and GSE115810. E-F. DCHS1 was lowly expressed in EC tissues and cell lines analyzed by qRT-PCR. Total RNA was isolated from 8 pairs of UCEC tissues and cell lines. G-H. DCHS1 differential expression in individual cancer stages and tumor grades via UALCAN database. I-K. The relationship between DCHS1 expression and HIPPO, WNT and mTOR pathways by using UALCAN online database. L. The silencing efficiency of siRNAs transfection into ISK and AN3CA cells analyzed by qRT-PCR. M. CCK8 analysis of DCHS1 silencing in ISK and AN3CA. N. Colony formation assay of the effect of DCHS1 knockdown on proliferation. O. Transwell assay of the invaded cells after DCHS1 silencing. P. The overexpression efficiency of pcDNA3.1-DCHS1 and pcDNA3.1 transfection into ISK and AN3CA cells analyzed by qRT-PCR. Q. CCK8 analysis of DCHS1 overexpression in ISK and AN3CA. R. Colony formation assay of the effect of DCHS1 overexpression on proliferation. S. Transwell assay of the invaded cells after DCHS1 overexpression. T. WB detecting the effect of DCHS1 silencing or overexpression on EMT markers. *p < 0.05, **p < 0.01, ***p < 0.001