Fig. 1

Workflow for the identification of overexpressed GPCRs in ovarian cancer tissues. The following stages were involved: Collection of RNA-seq raw data, removal of genes with no or very low expression based on CPM values, followed by TPM and RPKM quantification. Subsequent analysis was focused on genes with expression levels of at least 40% relative to the SDHA housekeeping gene, assessing expression frequencies within datasets and co-expression patterns between datasets (Venn analysis). The pre-selected candidate GPCRs were run through a final selection excluding candidates with high expression in healthy tissue samples. The remaining candidate GPCRs were validated using RT-qPCR and flow cytometry with primary ovarian cancer ascites cells and ovarian cancer cell lines