Fig. 10

Mitochondrial Biogenesis Status. a Relative Expression Level of Gene PGC1α by real time PCR. Results were normalized at first with GAPDH and then to the Control. Data are presented as mean ± standard deviation. a = P ≤ 0.002 vs MenSCs-EXO + PCOS + Control; b = P ≤ 0.0001 vs MenSCs-EXO + PCOS + Control and c = P ≤ 0.004 vs PCOS. b Immunofluorescence Staining of PGC1α (green) was performed in granulosa cells. The nuclei were stain with DAPI (blue). 400 × magnification; bars = 20 μm. c Semi-quantification of Mean Fluorescence Intensity of PGC1α in granulosa Cells following treatment with MenSCs-EXO and MenSCs. Data are presented as mean ± standard deviation. a = P ≤ 0.0001 vs MenSCs-EXO + PCOS + Control; b = P ≤ 0.044 vs PCOS. d Relative mtDNA Copy Number in Granulosa Cells of different groups were measured by quantitative real-time PCR (qPCR) and reported as a ratio of mitochondrial DNA (mt-DNA) to the nuclear DNA (nDNA). Data are presented as mean. a = P ≤ 0.001 vs MenSCs-EXO; b = P ≤ 0.0001 vs PCOS + Control and c = P ≤ 0.006 vs Control and d = P ≤ 0.004 vs PCOS