Fig. 4

Morphological characteristic and identification of Menstrual Blood-Derived Mesenchymal Stem Cells (MenSCs). a Morphology of passage 4–6 MenSCs observed under an inverted microscope showing spindle-shaped cells. 40 × magnification; bars = 200 μm. b Surface marker expression profile of MenSCs analyzed by flow cytometry; these cells were positive for CD105, CD90, and negative for CD34 and CD45, confirming their mesenchymal stem cell identity