Fig. 10

Effect of BM-MSCs transplantation on the expression of ovarian histopathological findings of the POI rats (A – R). Representative H&E images of CON (A, B, C, D, E, F), POI (G, H, I, J, K, L), and POI + BM-MSCs (M, N, O, P, Q, R) rat ovarian tissue. Control group: B: Squamous germinal epithelium (SG) and thin tunica albuginea (Tn). Cortex (C) with loose connective tissue stroma (CLS) housing primordial follicles comprising primary oocyte with an eccentric pale nucleus and cytoplasm enclosed by simple squamous granulosa cells (Sq). Primary follicle (PF) containing primary oocyte with a large pale nucleus and cytoplasm enclosed by simple cuboidal granulosa cells (CG). C: Secondary follicle composed of stratified granulosa cells with mitotic figures (MG), well-defined, eosinophilic zona pellucida (ZP), small liquor folliculi-filled clefts (LF) and theca folliculi (TF). D: Tertiary follicle with single antrum (An), stratum granulosum (SG) enclosed by large pale staining cells of theca interna (TI) and fibrous theca externa (TE). E: Corpora lutea (CL) showing large eosinophilic granulosa lutein cells (GL) intermingled with small intensely stained theca lutein cells (TL). F: Ovarian medulla (M) demonstrates richly vascularized (V) loose CT stroma (MLS). POI Group: H: Cuboidal germinal epithelium (Cu), thick tunica albuginea (Tk), cortical interstitial stroma hypercellularity (CHC), degenerated primordial (DPr), primary (DP), I: secondary and, J: tertiary follicles with indistinct zona pellucida (IZ), pycnotic (PG), shrunken and loosely arranged granulosa (LG) and theca interna cells (LI) and granular antral fluid (GA). K: Degenerated CL shows irregular-shaped granulosa lutein cells with pyknotic nuclei separated by large gaps (DGL). L: Medullary ovarian Hypercellular (MHC) with thickened, congested blood vessels (CV). POI + BM-MSCs group: N: Tunica albuginea of intermediate thickness (Im) and mild cortical interstitial stroma hypercellularity (MCC). O, P, Q: Nearly normal follicles and corpus luteum. R: Mild medullary cellularity (MMC) with mild vascular congested blood vessels (MVC). Scale bars A, G, M = 400 μm; B, C, D, E, H, I, J, K,N, O, P, Q = 40 μm, F, L, R = 150 μm