Fig. 9

Effect of BM-MSCs transplantation on the expression of ovarian anti-angiogenic VEGF-A 165b isofrm of the POI rats (A – G). Representative images from the anti-angiogenic VEGFA 165b isoform immunostained rat ovarian section of the CON (A), POI (C), and POI + BM-MSCs groups (E) and their respective higher magnifications (B, D, F). Notice faint (arrow), intense (arrowhead), and moderate (biforked arrow) cytoplasmic immunoreactivities in the granulosa lutein cells of the CON, POI, and POI + BM-MSCs groups, respectively. Scale bars A, C, E = 200 μm; B, D, F = 50 μm, and G. Area % of ovarian anti-angiogenic VEGFA 165b isoform positive immunostaining. The values represent the average of 6 to 8 rats in each group ± SEM *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001