Table 1 PCR primer and stem-loop primers for microRNA sequences
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Accession No | Product size |
|---|---|---|---|---|
Kiss-1 | TGCTGCTTCTCCTCTGTGTGG | ATTAACGAGTTCCTGGGGTCC | NM_181692.1 | 110 |
Kiss-1r | CTTTCCTTCTGTGCTGCGTA | CCTGCTGGATGTAGTTGACG | NM_023992.1 | 102 |
GnRH1 | AGGAGCTCTGGAACGTCTGAT | AGCGTCAATGTCACACTCGG | NM_012767.2 | 100 |
GnRHr | TCAGGACCCACGCAAACTAC | CTGGCTCTGACACCCTGTTT | NM_031038.3 | 182 |
GnIH | AGAGCAACCTAGGAAACGGGTGTT | AGGACTGGCTGGAGGTTTCCTATT | NM_023952.1 | 84 |
Star | CCCAAATGTCAAGGAAATCA | AGGCATCTCCCCAAAGTG | NM_031558.3 | 187 |
CYP11A1 | AAGTATCCGTGATGTGGG | TCATACAGTGTCGCCTTTTCT | NM_017286.3 | 127 |
CYP17A1 | TGGCTTTCCTGGTGCACAATC | TGAAAGTTGGTGTTCGGCTGAAG | NM_012753.2 | 90 |
CYP19A1 | GCTGAGAGACGTGGAGACCTG | CTCTGTCACCAACAACAGTGTGG | NM_017085.2 | 178 |
SRY | CTAGAGCTGCACACCAGTCC | Tgggtatccagtggggatgt | NM_001409365.1 | 77 |
IGF-1 | AAGCCTACAAAGTCAGCTCG | GGTCTTGTTTCCTGCACTTC | NM_001082477.2 | 166 |
GLP-1 | CACCTCCTCTCAGCTCAGTC | CGTTCTCCTCCGTGTCTTGA | NM_012707.2 | 128 |
PPAR-α | GTCCTCTGGTTGTCCCCTTG | GTCAGTTCACAGGGAAGGCA | NM_013196.2 | 176 |
PDCD4 | CGGCCCGAGGGGATTCTAAA | GGGTCAGTGGGGTTCACATT | NM_022265.3 | 123 |
PTEN | ATACCAGGACCAGAGGAAACC | TTGTCATTATCCGCACGCTC | NM_031606.2 | 101 |
notch-1 | CCTTGCTCTGCCTAACGCT | ATTCAGGCAGGTCCCACTTG | NM_001105721.1 | 80 |
p53 | CCCCTGAAGACTGGATAACTGT | TCTCCTGACTCAGAGGGAGC | NM_030989.3 | 75 |
BCL-2 | GACTGAGTACCTGAACCGGCATC | CTGAGCAGCGTCTTCAGAGACA | NM_016993.1 | 135 |
Hotair-1 | CCTTATAAGCTCATCGGAGCA | CATTTCTGGGTGGTTCCTTT | XR_005503470.1 | |
NEAT-1 | AAGGCACGAGTTAGCCGCAAAT | TGTGCACAGTCAGACCTGTCATTC | XR_005494740.1 | |
mir-34a | AACACGCTGGCAGTGTCTTA | GTCGTATCCAGTGCAGGGT | ||
mir-21 | AAGCGACCTAGCTTATCAGACT | GTCGTATCCAGTGCAGGGT | ||
mir-125a | AACAAGTCCCTGAGACCCTTTA | GTCGTATCCAGTGCAGGGT | ||
mir-664-5p | AACAAGCTGGCTGGGGAAA | GTCGTATCCAGTGCAGGGT | ||
mir-144-5p | AGCCAGCGGGATATCATCATATA | GTCGTATCCAGTGCAGGGT | ||
U6 | GCTCGCTTCGGCAGCACA | GAGGTATTCGCACCAGAGGA | ||
Stem-loop primers for microRNA | ||||
mir34a | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAACC | |||
mir125a | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAG | |||
mir-664 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCAATC | |||
mir-144-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTTAC | |||
mir-21-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAACA | |||