Fig. 7

STE exposure induces ferroptosis and reduces pERK1/2 expression in mouse oocytes. (A) Representative images of Fe²⁺ fluorescence in control and STE-exposed oocytes (scale bar, 20 μm). (B) Quantitative analysis of Fe²⁺ fluorescence intensity in control and STE-exposed oocytes (control group, control group, 32.3 ± 3.86 [number of replicates = 3, number of oocytes = 33, and number of mice = 3]; STE group, 19.0 ± 1.63 [number of replicates = 3, number of oocytes = 36, and number of mice = 3]). (C) Expression of GPX4 levels in control and STE-exposed oocytes. Proteins from 200 oocytes were loaded for each sample. (D) Relative-intensity results for GPX4 protein expression in the control and STE-treated oocytes. (E) Expression of ACSL4 levels in control and STE-exposed oocytes. Proteins from 200 oocytes were loaded for each sample. (F) Relative-intensity results for ACSL4 protein expression in the control and STE-exposed oocytes. (G) Representative images of GSH fluorescence in control and STE-exposed oocytes (scale bar, 80 μm). (H) Quantitative analysis of GSH fluorescence intensity in control and STE-exposed oocytes (control group, 31.7 ± 2.87 [number of replicates = 3, number of oocytes = 35, and number of mice = 3]; STE group, 16.0 ± 2.94 [number of replicates = 3, number of oocytes = 33, and number of mice = 3]). Experiments were repeated three times, and the results are presented as mean ± SD (*significantly different at p < 0.05 and **significantly different at p < 0.01)