Fig. 2

Effects of SCM-198 on spindle assembly and chromosome alignment in postovulatory aged oocytes. (A) Chromosome arrangement and spindle apparatus morphology of oocytes from different groups are shown. Oocytes were immunostained with α-tubulin-FITC antibody to visualize the spindles, and were counterstained with Hoechst to visualize the chromosomes. Scale bar = 20 μm. (B) Proportions of abnormal spindle assembly in the control, aged, and SCM-198-supplemented oocytes. (C) Proportions of misaligned chromosomes in the control, aged, and SCM-198-supplemented oocytes. Three parallel experiments were conducted, using the average ± SEM to represent the data of (B) and (C). **P < 0.01