Fig. 3

Effects of SCM-198 on the fertilization and sperm-egg binding ability of postovulatory aged oocytes. (A) Pictures of two-cell embryos obtained from different groups. Scale bar = 200 μm. (B) Proportions of in vitro fertilization rates in different groups. (C) Representative images of sperm binding to matured oocytes or two-cell embryos from different groups. Scale bar = 20 μm. (D) Schematic diagram of the results of counting the number of sperm adhered to the surface of ZP. Three parallel experiments were conducted, using the average ± SEM to represent the data of (B) and (D). *P < 0.05, **P < 0.01, and ***P < 0.001