Fig. 5

Effects of SCM-198 on the localization of Juno in postovulatory aged oocytes. (A) Representative images of Juno positioning images in different groups. Scale bar = 20 μm. (B) Schematic representation of Juno signal fluorescence intensity of control, aged, and SCM-198-supplemented oocytes. (C) Relative mRNA expression of Juno in different groups. (D) Western blotting was used to detect Juno protein expression levels in different groups. (E) Quantitative analysis of relative protein expression. Three parallel experiments were conducted using the average ± SEM to represent the data. *P < 0.05, **P < 0.01, ***P < 0.001