Fig. 6

Effects of SCM-198 on mitochondrial distribution, ROS accumulation, and apoptosis following aging for 24 h of treatment in vitro. (A) Representative images of mitochondrial distribution in control, aged, and SCM-198-supplemented oocytes. Scale bar = 20 μm. (B) The proportion of abnormal mitochondrial morphology in the control, aged, and SCM-198 groups. (C) Mitochondrial fluorescence intensity in the control, aged, and SCM-198-supplemented groups. (D) ROS staining results of different groups of sample oocytes. Scale bar = 20 μm. (E) ROS fluorescence signal detection results for different groups of sample oocytes. (F) Photographs of early apoptosis in Annexin V-stained oocytes from control, aged, and SCM-198-supplemented oocytes. Scale bar = 20 μm. (G) The detection results of Annexin V fluorescent signals intensity in different groups of sample oocytes. Three parallel experiments were conducted using the average ± SEM to represent the data of (B), (C), (E), and (G). *P < 0.05, **P < 0.01, ***P < 0.001