Fig. 7

Effect of SCM-198 supplementation on ROS levels, spindle assembly, and chromosome alignment in vivo. (A) Representative images of ROS levels in young, aged, and 15-day SCM-198 treatment groups of sample oocytes. Scale bar = 20 μm. (B) The fluorescence intensity of ROS signals was recorded in different groups of sample oocytes. (C) Representative images of ROS accumulation in young, aged, and 30-day SCM-198 treatment groups of sample oocytes. Scale bar = 20 μm. (D) ROS fluorescence signal detection results for different groups of sample oocytes. (E) Representative photos of spindle assembly and chromosome alignment in young and aged oocytes, as well as those supplemented with SCM-198 for 30 days. Oocytes were subjected to immunostaining using anti-α-tubulin-FITC antibody for visualizing spindles; counterstaining with Hoechst was performed to visualize chromosomes. Scale bar = 20 μm. (F) Ratios of abnormal spindle assembly in young and aged oocytes, as well as those supplemented with SCM-198 for 30 days. (G) Proportions of misaligned chromosomes in the young and aged oocytes, as well as those supplemented with SCM-198 for 30 days. Three parallel experiments were conducted using the average ± SEM to represent the data of (B), (D), (F), and (G). ***P < 0.001