Fig. 1

YJZYD improved ovarian function in DOR mice. A: In vivo experimental protocol in mice; B: Vaginal smear detection for estrus cycle; C-F: ELISA detection of serum AMH, FSH, LH, and E2 levels; G-H: HE staining to observe the morphology of the ovaries and compare changes in the number of follicles; I: TUNEL staining and FSHR co-labeling to assess apoptosis of ovarian GCs in ovarian tissues. n = 6. Data were expressed as mean ± SD. Multiple-group comparisons were statistically analyzed using one-way ANOVA, and post hoc tests were conducted using Tukey’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001