Fig. 2

YJZYD facilitated the proliferation of 4-HC-treated KGN and inhibited cell apoptosis. A: CCK-8 detection of cell proliferation; B: Flow cytometry analysis of cell apoptosis. The X-axis and Y-axis values in the flow chart refer to the fluorescence intensity of Annexin V and PI. The flow diagram was divided into four quadrants, with upper left quadrant Q1 (Annexin V⁻/PI⁺) representing mechanically damaged cells, upper right quadrant Q2 (Annexin V⁺/PI⁺) denoting late apoptotic cells, lower right quadrant Q3 (Annexin V⁺/PI⁻) representing early apoptotic cells, and lower left quadrant Q4 (Annexin V⁻/PI⁻) indicating normal/viable cells. The apoptotic rate was the sum of the percentage of Q2 and Q3 quadrants; C: Western blot to determine the expression levels of apoptosis-related proteins (Bax, Cleaved-caspase-3, and Bcl-2). Data were expressed as mean ± SD. Multiple-group comparisons were statistically analyzed using one-way ANOVA, and post hoc tests were conducted using Tukey’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001