Fig. 3

YJZYD suppressed mitochondrial fission and stimulated mitochondrial fusion in 4-HC-treated KGN cells. A-B: MitoTracker staining to detect the number of mitochondrial fragments and count the number of mitochondria; C: Western blot to measure protein expression levels of mitochondrial fission-related proteins (Drp1, p-Drp1 Ser616) and mitochondrial fusion-related proteins (MFN1, MFN2). Data were expressed as mean ± SD. Multiple-group comparisons were statistically analyzed using one-way ANOVA, and post hoc tests were conducted using Tukey’s multiple comparison test. ns represented P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001