Fig. 4

YJZYD regulated mitochondrial dynamics, proliferation, and apoptosis in 4-HC-treated KGN cells by activating the MAPK/ERK pathway. A: Western blot to measure the expression levels of ERK1/2 and p-ERK1/2 in KGN cells; B: CCK-8 detection of cell proliferation; C: Flow cytometry analysis of cell apoptosis; D: Western blot to assess the expression levels of apoptosis-related proteins (Bcl-2, Bax, and Cleaved-caspase-3); E-F: MitoTracker staining to detect the number of mitochondrial fragments and count the number of mitochondria; G: Western blot to test protein expression patterns of mitochondrial fission-related proteins (Drp1, p-Drp1 Ser616) and mitochondrial fusion-related proteins (MFN1, MFN2). Data were expressed as mean ± SD. Multiple-group comparisons were statistically analyzed using one-way ANOVA, and post hoc tests were conducted using Tukey’s multiple comparison test. ns represented P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001