Fig. 5

Downregulated SNHG18 inhibited cell viability and induced apoptosis of granulosa cells. A. Decreased expression of SNHG18 in SNHG18-knockdown SVOG cells detected by RT-qPCR. B. Annexin V-FITC/PI staining and flow-cytometry analysis of the proportion of apoptotic cells. C. TUNEL staining of the apoptotic cells. TUNEL-positive cells, red; cell nuclei, blue. Scale bars, 50 μm. (D) CCK8 assay of the SNHG18-knockdown SVOG cells. (E) EdU staining of the proliferative cells in SNHG18-knockdown SVOG cells. EdU-positive cells, red; cell nuclei, blue. Scale bars, 50 μm. (F) γ-H2A.X immunofluorescence of the SNHG18-knockdown SVOG cells. γ-H2A.X, pink; cell nuclei, blue. Scale bars, 10 μm. All of the experiments were performed in triplicate at least and the data were expressed as the mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001