Fig. 7

Downregulated SNHG18 by inhibited PKCε activation interfered with glycolysis via regulating ENO1 subcellular expression. A. ATP detection of SNHG18-knockdown SVOG cells. B. ECAR level detection of SNHG18-knockdown SVOG cells. C. OCR level of SNHG18-knockdown SVOG cells. D. The expression of ENO1 mRNA in SNHG18-knockdown SVOG cells. E. Western blotting detection of the total ENO1 level of SNHG18-knockdown SVOG cells. F. ENO1 detection by RT-qPCR in human primary granulosa cells from control, aged and POI women. G. The correlation between SNHG18 expression and ENO1 mRNA expression. H. ENO1 immunofluorescence in 13-week and 52-week mouse ovaries. ENO1, red; FOXL2, green; DDX4, yellow; DAPI, grey. I. ENO1 immunofluorescence of SNHG18-KD SVOG cells. ENO1, green; DAPI, blue. Scale bars, 10 μm. J. Western blotting detection of the nucleus and cytoplasm ENO1 level of SNHG18-knockdown SVOG cells. All of the experiments were performed in triplicate at least and the data were expressed as the mean ± SD. **, P < 0.01; ****, P < 0.0001