Fig. 1

NAC supplementation reverses estrous cycle irregularities and ovarian phenotypes in PCOS mice. A Schematic diagram of the animal study, where C57BL/6 female mice were administered letrozole (LE) by gavage to establish a PCOS model, and subsequently randomly divided into intervention groups. B-E Representative estrous cycle curves for the pure control group, PCOS + Met, PCOS + NAC, and the pure PCOS group during the intervention period (n = 12). F-I Representative ovarian histopathological images for the pure control group, PCOS + Met, PCOS + NAC, and the pure PCOS group after 12 days of intervention (n=6). Scale bar = 200 μm. LE, letrozole; Met, metformin; NAC, N-acetylcysteine; CMC, carboxymethyl cellulose