Fig. 2

Effects of NAC supplementation on endocrine and metabolic profiles in LE-induced PCOS mice. Serum levels of (A) follicle-stimulating hormone (FSH), (B) luteinizing hormone (LH), (C) testosterone (T), and (D) LH/FSH ratio were compared among different groups of mice after 12 days of intervention. Glucose tolerance test (E) and insulin tolerance test (G) results were also compared at the end of the intervention, with (F) and (H) representing the area under the curve for GTT and ITT, respectively. Infrared thermographic images (I) and core body temperature (J) for each group of mice at the conclusion of the study, along with body weight change curves (K) for each group throughout the study. Data are presented as mean ± SD and analyzed using one-way ANOVA with Tukey’s post hoc test (n = 4-6). *, p < 0.05; **, p < 0.01; ***, p < 0.001. Met, metformin; NAC, N-acetylcysteine