Fig. 6

Transcriptomic analyses and differentially expressed genes (DEGs) during summer and winter of granulosa cells in beef cows. Principal component analysis (PCA; A) and hierarchical heatmap (B) indicating the expression of genes in granulosa cells during summer and winter. Venn diagram (C) showing exclusively and mutually expressed genes between summer and winter and Volcano plot (D) indicating the differentially expressed genes from summer compared to the winter [Individual red and green dots represent the number of up- and downregulated genes, respectively (FC ≥ 2; FDR p-value ≤ 0.05)]. Summer = SUM (n = 4) and Winter = WIN (n = 4)