Fig. 6

NMN intervention downregulates the expression of pyroptosis factors induced by LPS and Nigricin trypanosomiasis in KGN cells. A) The levels of IL-1β and IL-18 in the supernatants were analyzed by enzyme-linked immunosorbent assay (ELISA); B) Relative mRNA expression of NLRP3, GSDMD, Caspase-1, IL-1β, and IL-18 in the KGN cells, analyzed using qRT-PCR; C) Protein expression of NLRP3, GSDMD, Caspase-1, IL-1β, and IL-18 in the KGN cells was determined using western blot analysis, β-tubulin served as the loading control; D) Relative protein expression of NLRP3, GSDMD, Caspase-1, IL-1β, and IL-18 in the KGN cells were quantified by ImageJ software and normalized to β-tubulin; E) The release of LDH in KGN cell detected by LDH activity assays. Data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001