Fig. 7

NMN enhances SIRT2 activation by elevating NAD⁺ levels to ameliorate KGN cell pyroptosis levels. A) Determine the content of NAD⁺ in KGN cells. B) Protein expression of SIRT2 in the KGN cells was determined using western blot analysis,𝛽-tubulin served as the loading control; C) Relative protein expression of SIRT2in the KGN cells was quantified by ImageJ software and normalized to β-tubulin; D) Relative mRNA expression of SIRT2 in the KGN cells analyzed using qRT-PCR; E) Relative mRNA expression of NLRP3, GSDMD, Caspase-1, IL-1β, and IL-18 in the KGN cells, analyzed using qRT-PCR; F) Protein expression of NLRP3, GSDMD, Caspase-1, IL-1β, and IL-18 in the KGN cells was determined using western blot analysis, β-tubulin served as the loading control; J) Relative protein expression of NLRP3, GSDMD, Caspase-1, IL-1β, and IL-18 in the KGN cells were quantified by ImageJ software and normalized to β-tubulin; H) The release of LDH in KGN cell detected by LDH activity assays. Data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001