Table 1 Primer sequences used for the qRT-PCR analysis
Target gene | Primer sequence(5’-3’) | GeneBank Accession no. |
|---|---|---|
RAT NLRP3 | F: GAGCTGGACCTCAGACAATGC R: AGAACCAATGCGAGATCCTGACAAC | XM 006246457.4 |
RAT GSDMD | F: ATTGGCTCTGAATGGGATA R: CGGCACCAGTTCTCCA | NM 001400994.1 |
RAT CASPASE-1 | F: GACCGAGTGGTTCCCTCAAG R: GACGTGTACGAGTGGGTGTT | NM 012762.3 |
RAT IL-18 | F: CGACCGAACAGCCAACGAATCC R: GTCACAGCCAGTCCTCTTACTTCAC | NM 019165.2 |
RAT IL-1β | F: CCCTTGTCGAGAATGGGCAG R: GACCAGAATGTGCCACGGTT | NM 031512.2 |
RAT SIRT2 | F: CTCGCCTGCTCATCAACAAG R: GTCCTCCAGCTCCTTCTTCC | NM 001399630 |
RAT GAPDH | F: GAGTCCACTGGCGTCTTCAC R: GAGGCATTGCTGATGATCTTGAG | XM 032916238 |
HOMO NLRP3 | F: ATGCTGCTTCGACATCTCCT R: AACCAATGCGAGATCCTGAC | XM 047443562.1 |
HOMO GSDMD | F: CAGAAGGGACGTGGTGTTCC R: AAGTGTTGAGGGCAGAACCC | NM 001166237 |
HOMO IL-18 | F: ATGGCTGCTGAACCAGTAGAAGAC R: TCCGGGGTGCATTATCTCTACAGTC | NM 001562.4 |
HOMO IL-1β | F: CCGACCACCACTACAGCAAGG R: GGGCAGGGAACCAGCATCTTC | NM 000576.3 |
HOMO SIRT2 | F: CGCACGGCACCTTCTACACATC R: GGCTCTGACAGTCTTCACACTTGG | NM 001193286 |
HOMO GAPDH | F: GAGTCCACTGGCGTCTTCAC R: GAGGCATTGCTGATGATCTTGAG | M33197.1 |