Fig. 2
hUC-MSCs-EVs upregulate IGF-1 to improve mouse ovarian function in CTX-stimulated POI
A-B: H&E staining displayed ovarian histopathological changes, and the numbers of total follicles and follicles at all levels (including secondary, primordial, mature, primary and atretic follicles) were statistically analyzed; C: serum E2, FSH, LH, and AMH levels were detected by ELISA; D: immunohistochemistry was used to detect the expression of IGF-1. N = 6, and the data were expressed as mean ± standard deviation. Data comparisons among multiple groups were performed by one-way analysis of variance, followed by Tukey’s multiple comparisons test, **, P < 0.01; ***, P < 0.001. hUC-MSCs-EV, human umbilical cord mesenchymal stem cell-derived extracellular vesicles; E2, estradiol; FSH, follicle stimulating hormone; LH, luteinizing hormone; AMH, anti-mullerian tubular hormone