Fig. 3

hUC-MSCs-EVs carrying IGF-1 ameliorates CTX-induced damage of GCs

A: Western blot quantified the protein levels of IGF-1 carried in differently-treated EVs; B: RT-qPCR and western blot verified IGF-1 expression in hUC-MSCs; C: PKH26 staining was used to observe the uptake of hUC-MSCs-EVs by GCs; D: Western blot quantified IGF-1 expression in GCs; E: CCK-8 assay was used to evaluate GC proliferation; F: LDH determined GC death. The cellular experiments were independently repeated three times, and the data were expressed as mean ± standard deviation. One-way analysis of variance was used for data comparison among multiple groups, followed by Tukey’s multiple comparisons test. **, P < 0.01; ***, P < 0.001. hUC-MSCs-EV, human umbilical cord mesenchymal stem cell-derived extracellular vesicles; IGF-1, insulin-like growth factor 1; GCs, granulosa cells; LDH, lactate dehydrogenase