Fig. 3
From: ATF3 mediates PM2.5-induced apoptosis and inflammation in ovarian granulosa cells
PM2.5 exposure triggers oxidative stress and inflammation in vitro. (A) Heat map, Cutoff for logFC is 1. The number of up-regulated genes is 181, and down genes is 162. (B) Up-gene-KEGG-Analysis. (C-D) GSEA results. (E-F) Cell apoptosis rates in KGN cells were increased in a dose-dependent manner (n = 3). (G) The expression level of TNFα/IL-1β/IL-6 in cell supernatant was detected by ELISA (n = 2). (H-I) Using the fluorescent dye MitoSOX to study the generation of reactive oxygen species(ROS) (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001