Fig. 4

Effects of miR-34a-5p on glycolysis in PCOS cell models. The effect of testosterone treatment on KGN cell viability. B qRT-PCR analysis of the miR-34a-5p expression in KGN cells. C qRT-PCR analysis of the expression of key enzymes of glycolysis (HK2, PKM2, LDHA) in a cell model under a control environment. D qRT-PCR analysis of the expression of key enzymes of glycolysis (HK2, PKM2, LDHA) in cell models under “+ testosterone” environment. E Concentration of pyruvate in KGN cell culture medium. F Concentration of lactic acid in KGN cell culture medium. G Western blotting of LDHA in KGN cells. H Western blotting of HK2 in KGN cells. I Western blotting of PKM2 in KGN cells. *p < 0.05, **p < 0.01