Table 1 Primers for real-time quantitative PCR
Gene | 5′–3′ Sequence |
|---|---|
Homo Caspase-3 | F: CAGTGATGCTGTGCTATGAAT R: CAGATGCCTAAGTTCTTCCAC |
Homo Bax | F: GCGACTGATGTCCCTGTCT R: TGAGTGAGGCGGTGAGC |
Homo Bcl-2 | F: CCCTGTGGATGACTGAGTACC R: AGACAGCCAGGAGAAATCAAA |
Homo HK2 | F: CGAGAGCATCCTCCTCAAGTG R: AGCCACAGGTCATCATAGTTCC |
Homo PKM2 | F: TGGGAGAGAAGGGAAAGAACATC R: GCACCGTCCAATCATCATCTTC |
Homo LDHA | F: ATGAGTTGGACTGTGCCTGTTGTG R: GTGAAGAGCCAGGTGCCGTTG |
Homo GAPDH | F: GAGTCCACTGGCGTCTTCAC R: GAGGCATTGCTGATGATCTTGAG |
hsa-miR-34a-5p stem ring primer | F: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAACC R: AGTGCAGGGTCCGAGGTATT |
hsa-miR-34a-5p | F: CGCTGGCAGTGTCTTAGCT R: AGTGCAGGGTCCGAGGTATT |
U6 | F: GGAACGATACAGAGAAGATTAGC R: TGGAACGCTTCACGAATTTGCG |