Table 1 Sequences of primers for target genes and miRNA used
From: MSCs–derived EVs protect against chemotherapy-induced ovarian toxicity: role of PI3K/AKT/mTOR axis
Gene/miRNA | Sequence | Accession number | |
|---|---|---|---|
PTEN | Forward | AGACCATAACCCACCACAGC | NM_031606.2 |
Reverse | TCACCTTTAGCTGGCAGACC | ||
FOXO3 | Forward | GCCTCATCTCAAAGCTGGGT | NM_001106395.1 |
Reverse | AGTTCTGCTCCACGGGAAAG | ||
GAPDH | Forward | TGCTGGTGCTGAGTATGTCG | NM_017008.4 |
Reverse | TTGAGAGCAATGCCAGCC | ||
miR-200c | Forward | GTTTG CGTCTTACCCAGCA | MIMAT0017150 |
Reverse | GTGCAGGGTCCGAGGT | ||
miR-122 | Forward | GTGTGGAGTGTGACAATGG | MIMAT0000827 |
Reverse | GTGCAGGGTCCGAGGT | ||
miR-99 | Forward | GTGAACCCGTAGATCCGAT | MIMAT0000820 |
Reverse | GTGCAGGGTCCGAGGT | ||
U6 | Forward | CTCGCTTCGGCAGCACA | XR_004936894 |
Reverse | AACGCTTCACGAATTTGCGT | ||