Fig. 5

CPI-613 reverses the chemotherapy induced metabolic reprogramming in chemoresistant ovarian xenografts. Single cell suspension was prepared from freshly isolated (A) CaOV3 and (F) F2 xenografts (n = 3), cells were pooled to plate 70,000 cells/well in triplicates and subjected to real-time XFe Seahorse analysis for bioenergetics profiling. Oxygen consumption rate (OCR), an indicator for mitochondrial respiration, was assessed with port injections of (1) oligomycin, (2) FCCP, and a combination of (3) rotenone-antimycin. B, G The bar graph represents basal and stressed OCR. Targeted analysis of the TCA cycle metabolites was performed to assess the levels of various metabolites in pooled xenografts (n = 3) in triplicates. Key metabolites are shown as (C, H) acetyl CoA, (D, I) alpha- ketoglutarate (αKG), and (E, J) succinate. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 comparisons as indicated