Fig. 5

Immune Cell Infiltration Analysis in High-Grade Serous Ovarian Cancer (HGSOC) using TIMER 2.0. This figure illustrates the immune cell infiltration profiles for three cell types —Macrophages, B cells, and Cancer-Associated Fibroblasts (CAFs)—using multiple deconvolution methods in TIMER 2.0, with n = 303 (A, C, E). Heatmaps illustrating expression intensity of Macrophages (A), B cells (C), and CAFs (E) across different HGSOC samples based on several deconvolution methods: EPIC, TIMER, CIBERSORT, MCPCOUNTER, TIDE, QUANTISEQ and xCell. The color gradient found in the heatmap represents Spearman correlation values, with red indicating positive correlations and blue representing negative ones. Statistically significant correlations (p < 0.05) are marked. Significant variations in infiltration profiles are seen across samples and methods. B, D, F Scatter plots of gene expression levels (LAIR-1, COL3A1, COL1A1, FCRL5 and IRF4) vs. tumor purity and infiltration levels for each of the three immune cell types. Tumor purity is negatively correlated with immune infiltration, and these panels visualize the relationship between gene expression and estimated cell infiltration