Fig. 2
From: Single-cell sequencing reveals PTX3 involvement in ovarian cancer metastasis
PTX3 expression and function in OC cells. (A) Quantitative real-time PCR detection of PTX3 gene expression in ovarian epithelial cells and OC cells. (B) Western blot detection of PTX3 protein expression in ovarian epithelial cells and OC cells (left). Quantitative analysis of PTX3 (right). (C, D) Western blot detection of PTX3 protein expression in control and PTX3 knockdown OVCAR3 and CAOV3 cells (left). Quantitative analysis of PTX3 (right). (E, F) Western blot showing PTX3 protein expression in control and PTX3 overexpression SKOV3 and CAOV3 cells (left). Quantitative analysis of PTX3 (right). (G-J) EDU assay detecting the effects of PTX3 knockdown and overexpression on the proliferation of OC cells. Scale bar, 100 μm. (K-L) CCK8 assay detecting the effects of PTX3 knockdown and overexpression on the proliferation of OC cells. Data are presented as mean ± SD; ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001