Fig. 5

PTX3 activates EMT and NF-κB pathways in OC cells, partially inhibited by TLR4. (A, B) IF staining results of OVCAR3 cells, showing PTX3 (green), TLR4 (red), CD44 (red), and the nuclear marker DAPI (blue). Scale bar, 100 μm. (C, D) Co-IP detected the interaction of PTX3 with TLR4 and CD44 in OVCAR3 cells. (E, F) Representative Western blot images showing the expression of E-cadherin, N-cadherin, and vimentin after PTX3 knockdown (upper) Quantitative analysis of the respective proteins (lower). (G, H) Representative Western blot images showing the expression of E-cadherin, N-cadherin, and vimentin after PTX3 overexpression and after PTX3 overexpression with the addition of TLR4 inhibitor (upper). Quantitative analysis of the respective proteins (lower). (I, J) Representative Western blot images showing the expression of NF-κB and p-NF-κB in OC cells after PTX3 knockdown (upper). Quantitative analysis of the respective proteins (lower). (K, L) Representative Western blot images showing the expression of NF-κB and p-NF-κB in OC cells after PTX3 overexpression and after PTX3 overexpression with the addition of TLR4 inhibitor (upper). Quantitative analysis of the respective proteins (lower). Data are presented as mean ± SD; ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001